Fluorescence-based detection of single-nucleotide changes in RNA using graphene oxide and DNAzyme.
نویسندگان
چکیده
We report a simple fluorometric method for detection of single-nucleotide changes in RNA using graphene oxide (GO) and RNA-cleaving DNAzyme. The fluorescent DNA probe (F-DNA) was annealed to RNA fragments generated by RNA cleavage with DNAzyme specific to mutant RNA. The F-DNA-RNA duplex attenuated the quenching of F-DNA fluorescence by GO.
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ورودعنوان ژورنال:
- Chemical communications
دوره 51 26 شماره
صفحات -
تاریخ انتشار 2015